Development of Polymeric Nanoparticles of Garcinia mangostana Xanthones in Eudragit RL100/RS100 for Anti-Colon Cancer Drug Delivery

Table 7

Ease of reconstitution of the freeze-dried NPs. The freeze-dried cakes were reconstituted in the same volume of water and were given a score as the following: reconstituted immediately (++++), reconstituted after several manual inversions for 1 min (+++), reconstituted after vortex for 1 min (++), reconstituted after sonication (+), and not reconstituted after sonication (0).

Cryoprotectant	Formulations
	Ease of reconstitution
	Tween 80		Tween 20		PVA		None
	0.1%	0.05%	0.1%	0.05%	0.1%	0.05%
Sucrose	++	+	++	++	+	+	0
Glucose	++	++	++	+++	+++	+++	0
Mannitol	0	0	0	0	0	0	0
Glycine	0	0	0	0	0	0	0
Glycerol	+++	++	+	+++	+++	+++	0
Dextran 40000	0	0	0	0	0	0	0
None	0	0	0	0	0	0	0