Autophagy in RAW264.7 Cells Treated with Surface-Functionalized Graphene Oxides

Figure 4

Expression of GAPDH, p-Act, PU.1, and autophagy marker proteins in RAW264.7 cells treated with the different concentrations of GO types for different treatment durations. (a) Representative results of western blot analysis of each protein. (b) Changes in the ratio of LC3A/B-II/(LC3A/B-I + LC3A/B-II) as a function of GO concentration and treatment duration. Level of LC3A/B-I and LC3A/B-II expression was analyzed by western blot analysis by using specific antibodies.

(a) GO

(b) DA-GO

(c) rGO

(d) SDS-rGO