Surface Epitope Coverage Affects Binding Characteristics of Bisphenol-A Functionalized Nanoparticles in a Competitive Inhibition Assay

Table 2

Assay parameters and calculated affinity constant derived from Figure 3.

Nanoparticle	265 (●)	265 (□)	265 (▲)
Particle number (mol L^−1)	6.6 × 10^−10	3.3 × 10^−10	1.3 × 10^−10
Molar concentration of surface epitope (mol L^−1)	1.7 × 10^−7	9.0 × 10^−8	3.0 × 10^−8
Slope from Figure 3(b)	1.1 ± 0.11	0.87 ± 0.07	0.88 ± 0.13
IC50 (µmol L^−1)	8.0 ± 1.5	4.4 ± 1.2	1.2 ± 1.2
Affinity constant (, µmol^−1)	0.13 ± 0.02	0.23 ± 0.03	0.62 ± 0.09
Linear detection range (µmol L^−1)	1.9–30.2	0.9–18.6	0.2–5.6

20–80% of the % curve.