Research Article
Surface Epitope Coverage Affects Binding Characteristics of Bisphenol-A Functionalized Nanoparticles in a Competitive Inhibition Assay
Table 3
Assay parameters and calculated affinity constant derived from Figure
4.
| Nanoparticle | 190 (●) | 396 (□) | 801 (▲) |
| Particle number (mol L−1) | 2.1 × 10−10 | 2.1 × 10−10 | 2.1 × 10−10 | Molar concentration of surface epitope (mol L−1) | 4.0 × 10−8 | 8.0 × 10−8 | 1.7 × 10−7 | Slope from Figure 4(b) | 0.68 ± 0.08 | 0.81 ± 0.10 | 0.36 ± 0.14 | IC50 (µmol L−1) | 0.7 ± 1.2 | 1.8 ± 1.2 | 20.0 ± 15.4 | Affinity constant (, µmol−1) | 1.1 ± 0.2 | 0.55 ± 0.08 | 0.34 ± 0.10 | Linear detection range (µmol L−1) | 0.09–5.2 | 0.32–11.8 | 0.20–44.7 |
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20–80% of the % curve.
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