Controlling Surface-Induced Nanocomposites by Lipoplexes for Enhanced Gene Transfer

Figure 5

The cellular DNA uptake mediated by surface-induced lipoplex-nanocomposites prepared from mineralizing solutions, A, B, G, H, G-Sr, and G-F. Naked DNA-doped nanocomposites (naked DNA) and free lipoplexes made from DOTAP (DOTAP) were used as controls. The DOTAP liposomes were prepared using freeze-thaw-extrusion method with 100 nm or 1000 nm filter. Fluorescein-labeled DNA was used. The ratio of DNA to DOTAP was 1 : 12 (w/w). Data were shown as means ± s.e. Statistical significance of differences was determined by the unpaired two-tailed Student’s -test. The uptake mediated by lipoplex-nanocomposites was considered statistically significantly different from that by DNA-nanocomposites at .