Controlling Surface-Induced Nanocomposites by Lipoplexes for Enhanced Gene Transfer

Figure 6

The gene transfer efficiency in B35 cells medicated by surface-induced lipoplex-nanocomposites. (a) The effect of liposome sizes. DNA : DOTAP ratio was 1 : 12 (w/w); the complexation duration was 15 min. (b) The effect of DNA : DOTAP ratios. The complexation duration was 15 min; the filter size is 1000 nm. The DOTAP liposomes were prepared using freeze-thaw-extrusion method. Naked DNA-doped nanocomposites (naked DNA) and free lipoplexes prepared using DOTAP (DOTAP) were used as controls. (c) The lipoplexes were made from Lipofectamine 2000. The DNA was complexed with Lipofectamine 2000 at different ratios and the complexation duration was 20 min. Data were shown as means ± s.e. Statistical significance of differences was determined by the unpaired two-tailed Student’s -test. The gene transfer efficiency mediated by lipoplex-nanocomposites was considered statistically significantly different from that by DNA-nanocomposites at , , and .

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