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Journal of Nanomaterials
Volume 2015, Article ID 789089, 7 pages
Research Article

Extracellular Biosynthesis of Copper and Copper Oxide Nanoparticles by Stereum hirsutum, a Native White-Rot Fungus from Chilean Forests

1Doctoral Program of Science of Natural Resources, Universidad de La Frontera, Chile
2Scientific and Technological Bioresource Nucleus, Instituto Química, Universidad de La Frontera, Chile
3Biological Chemistry Laboratory, Instituto Química, Universidade Estadual de Campinas, SP, Brazil
4Department of Chemical Engineering, Universidad de La Frontera, Chile

Received 29 July 2014; Revised 16 December 2014; Accepted 30 December 2014

Academic Editor: Ronen Hazan

Copyright © 2015 R. Cuevas et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The white-rot fungus Stereum hirsutum was studied to evaluate its applicability for use in the biosynthesis of copper/copper oxide nanoparticles under different pH conditions and in the presence of three different copper salts (CuCl2, CuSO4, and Cu(NO3)2). The nanoparticle formation was evaluated by UV-visible spectroscopy, electron microscopy (TEM), X-ray diffraction analysis (XRD), and Fourier transforms infrared spectroscopy (FTIR). The nanoparticles biosynthesis in presence of all copper salts demonstrated higher formation with 5 mM CuCl2 under alkaline conditions. TEM analysis confirmed that the nanoparticles were mainly spherical (5 to 20 nm). The presence of amine groups attached to nanoparticles was confirmed by FTIR, which suggests that extracellular protein of fungus is responsible for the formation of the nanoparticles. Therefore, the white-rot fungus S. hirsutum was found to exhibit potential for use in the synthesis of copper/copper oxide nanoparticles.