Development of Bioactive Patch for Maintenance of Implanted Cells at the Myocardial Infarcted Site

Figure 3

Bioimplant characterization. Microscopic images of subATDPCs growing in developed composite after 7 seeding days. (a) Surface of PEA scaffold; (b) surface of PCLMA scaffold; ((c), (d)) close up of top PEA, and PCLMA scaffold respectively; (e) PEA scaffold cross section; (f) PCLMA scaffold cross section; and ((g) and (h)) Dapi & Phalloidin staining of subATDPCs in PEA and PCLMA scaffold. In images (a) to (f) white arrows indicate the cells growing in the bioactive implant, black arrows note the nanofibers of self-assembling peptide RAD16-I, and red arrows signalize the structure of porous elastomeric membrane. White arrows in image (g) and (h) indicate scaffolds surface while orange arrows indicate cells migrating inside the composite. Scale bars: (a) 30 μm; (b), (E)–(H) 100 μm; (c) 3 μm; and (d) 5 μm.