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Journal of Nanomaterials
Volume 2015, Article ID 858493, 8 pages
Research Article

The Preparation and In Vitro Evaluations of a Nanoscaled Injectable Bone Repair Material

1Orthopaedics, The Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing 211100, China
2Orthopaedics, The First Hospital of Hebei Medical University, Shijiazhuang 050031, China
3Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, China

Received 19 September 2014; Revised 6 January 2015; Accepted 7 January 2015

Academic Editor: Shuming Zhang

Copyright © 2015 Xinhui Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


There are usually two forms of bone repair materials, block and granular, for common clinical use. This paper describes a novel injectable material, nano-HA/collagen/alginate (nHAC/Alg) composite biomaterial, including its preparation and evaluations in vitro. Based on the idea of bionics and the study of collagen/calcium phosphate salt composite materials, the injectable bone repair material was developed. Then, human bone marrow stem cells (hBMSCs) were cultured on the nHAC/Alg material. The cell attachment, proliferation, and differentiation were evaluated with inverted microscope, scanning electron microscope, laser scanning confocal microscope, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) analysis, and alkaline phosphatase (ALP) test. The results showed that nHAC/Alg not only had no negative effect on cellular functions but also promotes cell proliferation and differentiation into osteogenic cells, which suggests that the nanoscaled injectable bone repair material has good clinical application prospects for bone repair.