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| Description | Points to be addressed | Observations on the production site |
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| Hazard | (a) Number of sources emitting pollution | Three sources: extruder, storage, and garbage |
| (b) Size of the production and waste generation in relation to the production capacity | A daily intensive production (5 batches per day) produces 75 mL. If we consider 260 days/year of production, the maximum total production is 19,500 mL/year. The phospholipid concentration in dioleoylphosphatidylethanolamine (DOPE) is 6.43 mM (4.78 mg/mL) in the final product
The waste consists of residues from water based solution which is used to clean the extruder and tools |
| (c) Size of each of the sources | During extrusion and storage phases, 15 mL of nanoliposome suspension is dealt with |
| (d) Type and location of each source | (a) Nanoliposomes production on the three benches A, B, and C,
(b) fridge, (c) garbage (Figure 1) |
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| Probability of exposure | (a) Dispersion of pollutants by air movement | There was neither any measured dispersion nor any ATEX risk identified |
| (b) Proximity to sources/confinement barrier | Handling without hood |
| (c) Time passed in a location | 10 min for handling the nanoliposome liquid suspensions, 60 min for their extrusion, and 30 min for their purification |
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| Preventive measures | (a) Type and efficacy of evacuation and ventilation systems | Presence of hood for chemical and biological products; no measured dispersion |
| (b) Personal working habits | In accordance with the chemical/biological risk
No direct account taken yet of nanorisk |
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