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Journal of Nanomaterials
Volume 2015, Article ID 980529, 5 pages
Research Article

Intratubular Antibacterial Effect of Polyethyleneimine Nanoparticles: An Ex Vivo Study in Human Teeth

1Department of Endodontics, Hebrew University-Hadassah School of Dental Medicine, P.O. Box 12272, 91120 Jerusalem, Israel
2Department of Prosthodontics, Hebrew University-Hadassah School of Dental Medicine, P.O. Box 12272, 91120 Jerusalem, Israel
3Goldschleger School of Dental Medicine, Tel Aviv University, P.O. Box 39040, 6139001 Tel Aviv, Israel

Received 22 March 2015; Revised 11 May 2015; Accepted 12 May 2015

Academic Editor: Victor M. Castaño

Copyright © 2015 Itzhak Abramovitz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Enterococcus faecalis is a facultative gram positive bacterium which can remain in the teeth root canals and cause refractory or persistent periapical diseases. E. faecalis bacteria that penetrate the dentinal tubules can be the source of intracanal infection and endodontic disease. Quaternary ammonium polyethyleneimine (QPEI) nanopolymers were shown to have long lasting antibacterial activity against gram positive and gram negative bacteria. The present study evaluated the intratubular antibacterial effect of an epoxy resin sealer incorporating 1% QPEI against E. faecalis in a human dentin model. Root canals of extracted teeth were inoculated with E. faecalis for 7 days prior to standard endodontic treatment. The antibacterial effect of an epoxy-amine resin endodontic sealer was tested at concentration of 0% or 1% (wt/wt) added QPEI nanoparticles. Reduction in bacterial viability was depicted in the dentinal tubules of the root canals obturated with the sealer incorporating QPEI nanoparticles. In conclusion, QPEI nanoparticles when incorporated in a small percentage into epoxy-resin based sealer may target E. faecalis in the dentinal tubules, producing a potent antibacterial effect that reduces significantly bacterial viability.