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Journal of Nanomaterials
Volume 2016 (2016), Article ID 1730403, 9 pages
Research Article

Facile Method for Preparation of Silica Coated Monodisperse Superparamagnetic Microspheres

1Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea
2School of Chemical and Biological Engineering, Seoul National University, Seoul 151-747, Republic of Korea
3Department of Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea

Received 15 October 2015; Revised 28 December 2015; Accepted 10 January 2016

Academic Editor: Zhisong Lu

Copyright © 2016 Xuan-Hung Pham et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This paper presents a facile method for preparation of silica coated monodisperse superparamagnetic microsphere. Herein, monodisperse porous polystyrene-divinylbenzene microbeads were prepared by seeded emulsion polymerization and subsequently sulfonated with acetic acid/H2SO4. The as-prepared sulfonated macroporous beads were magnetized in presence of Fe2+/Fe3+ under alkaline condition and were subjected to silica coating by sol-gel process, providing water compatibility, easily modifiable surface form, and chemical stability. FE-SEM, TEM, FT-IR, and TGA were employed to characterize the silica coated monodisperse magnetic beads (~7.5 μm). The proposed monodisperse magnetic beads can be used as mobile solid phase particles candidate for protein and DNA separation.