Effects of Multiwalled Carbon Nanotube Surface Modification and Purification on Bovine Serum Albumin Binding and Biological Responses

Figure 2

Confirmation of BSA bound to MWCNTs. (a) Quantification of BSA bound to MWCNT using a Pierce BCA protein assay; (b) SDS-PAGE image of BSA binding to MWCNTs. Lanes: 1, BSA alone; 2, BSA bound to pristine MWCNT; 3, BSA bound to F-MWCNT; 4, BSA bound to BW-F-MWCNT. (c–e) UV-vis spectral analysis of MWCNTs with and without BSA coating. In (a) , compared to the amount of BSA bound to MWCNT; , significant difference of the amount of BSA between F-MWCNT and BW-F-MWCNT.

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