Extracellular Biofabrication, Characterization, and Antimicrobial Efficacy of Silver Nanoparticles Loaded on Cotton Fabrics Using Newly Isolated Streptomyces sp. SSHH-1E

Table 3

Full-factorial Plackett-Burman design matrix of 14 independent (assigned) variables and mean response for biosynthesis of AgNPs by Streptomyces narbonensis SSHH-1E.

Run	Temperature; ()	pH; ()	Incubation; time ()	Inoculum; size ()	Inoculum; age ()	Agitation; speed ()	Medium; volume ()	Starch; ()	NaNO3; ()	K2HPO4; ()	MgSO4·7H2O; ()	Yeast extract; ()	Malt extract; ()	Peptone; ()	Absorbance at 400 nm
															Experimental	Predicted
1	1 (37)	−1 (6)	1 (7)	−1 (2)	−1 (24)	−1 (150)	−1 (50)	1 (20)	1 (3)	−1 (0.5)	1 (1)	1 (1)	−1 (1)	−1 (0.5)	0.03	−0.036
2	−1 (30)	1 (8)	1 (7)	−1 (2)	1 (48)	1 (200)	−1 (50)	−1 (10)	1 (3)	1 (1.5)	1 (1)	1 (1)	−1 (1)	1 (1)	0.80	0.746
3	1 (37)	−1 (6)	−1 (5)	−1 (2)	−1 (24)	1 (200)	1 (100)	−1 (10)	1 (3)	1 (1.5)	−1 (0.25)	−1 (0.5)	1 (2)	1 (1)	0.12	0.056
4	1 (37)	1 (8)	1 (7)	1 (4)	−1 (24)	1 (200)	−1 (50)	1 (20)	−1 (1)	−1 (0.5)	−1 (0.25)	−1 (0.5)	1 (2)	1 (1)	0.01	−0.043
5	1 (37)	−1 (6)	1 (7)	−1 (2)	1 (48)	−1 (150)	−1 (50)	−1 (10)	−1 (1)	1 (1.5)	1 (1)	−1 (0.5)	1 (2)	1 (1)	0.06	0.144
6	−1 (30)	−1 (6)	−1 (5)	−1 (2)	1 (48)	1 (200)	−1 (50)	1 (20)	1 (3)	−1 (0.5)	−1 (0.25)	1 (1)	1 (2)	1 (1)	0.17	0.206
7	1 (37)	1 (8)	−1 (5)	1 (4)	1 (48)	−1 (150)	−1 (50)	1 (20)	1 (3)	1 (1.5)	1 (1)	−1 (0.5)	1 (2)	−1 (0.5)	0.90	0.843
8	−1 (30)	1 (8)	1 (7)	−1 (2)	−1 (24)	1 (200)	1 (100)	1 (20)	1 (3)	−1 (0.5)	1 (1)	−1 (0.5)	1 (2)	−1 (0.5)	0.10	0.186
9	1 (37)	1 (8)	−1 (5)	1 (4)	−1 (24)	1 (200)	−1 (50)	−1 (10)	−1 (1)	−1 (0.5)	1 (1)	1 (1)	−1 (1)	1 (1)	0.21	0.262
10	−1 (30)	−1 (6)	−1 (5)	1 (4)	1 (48)	−1 (150)	1 (100)	1 (20)	−1 (1)	−1 (0.5)	1 (1)	1 (1)	1 (2)	1 (1)	0.20	0.161
11	−1 (30)	−1 (6)	1 (7)	1 (4)	−1 (24)	1 (200)	1 (100)	−1 (10)	−1 (1)	1 (1.5)	1 (1)	1 (1)	1 (2)	−1 (0.5)	0.01	−0.037
12	1 (37)	1 (8)	−1 (5)	−1 (2)	1 (48)	1 (200)	1 (100)	1 (20)	−1 (1)	1 (1.5)	−1 (0.25)	1 (1)	−1 (1)	−1 (0.5)	1.45	1.458
13	−1 (30)	−1 (6)	−1 (5)	−1 (2)	−1 (24)	−1 (150)	−1 (50)	−1 (10)	−1 (1)	−1 (0.5)	−1 (0.25)	−1 (0.5)	−1 (1)	−1 (0.5)	0.35	0.326
14	−1 (30)	1 (8)	1 (7)	1 (4)	1 (48)	−1 (150)	1 (100)	−1 (10)	1 (3)	−1 (0.5)	−1 (0.25)	−1 (0.5)	−1 (1)	1 (1)	1.40	1.375
15	1 (37)	−1 (6)	−1 (5)	1 (4)	1 (48)	1 (200)	1 (100)	−1 (10)	1 (3)	−1 (0.5)	1 (1)	−1 (0.5)	−1 (1)	−1 (0.5)	1.10	1.131
16	−1 (30)	1 (8)	−1 (5)	−1 (2)	−1 (24)	−1 (150)	1 (100)	1 (20)	−1 (1)	1 (1.5)	1 (1)	−1 (0.5)	−1 (1)	1 (1)	0.00	−0.002
17	−1 (30)	−1 (6)	1 (7)	1 (4)	1 (48)	1 (200)	−1 (50)	1 (20)	−1 (1)	1 (1.5)	−1 (0.25)	−1 (0.5)	−1 (1)	−1 (0.5)	0.58	0.590
18	1 (37)	−1 (6)	1 (7)	1 (4)	−1 (24)	−1 (150)	1 (100)	1 (20)	1 (3)	1 (1.5)	−1 (0.25)	1 (1)	−1 (1)	1 (1)	0.03	0.086
19	1 (37)	1 (8)	1 (7)	−1 (2)	1 (48)	−1 (150)	1 (100)	−1 (10)	−1 (1)	−1 (0.5)	−1 (0.25)	1 (1)	1 (2)	−1 (0.5)	1.60	1.592
20	−1 (30)	1 (8)	−1 (5)	1 (4)	−1 (24)	−1 (150)	−1 (50)	−1 (10)	1 (3)	1 (1.5)	−1 (0.25)	1 (1)	1 (2)	−1 (0.5)	0.60	0.653