Extracellular Biofabrication, Characterization, and Antimicrobial Efficacy of Silver Nanoparticles Loaded on Cotton Fabrics Using Newly Isolated Streptomyces sp. SSHH-1E
Table 4
Estimated regression coefficients for optimization of AgNPs biosynthesis using Plackett-Burman design.
Variables
Coefficients
Main effect
-stat
value
Confidence level (%)
Intercept
0.485
0.97
22.59
0.00
100.000
Temperature
0.064
0.13
3.00
0.03
96.995
pH
0.222
0.44
10.35
0.00
99.985
Incubation time
−0.025
−0.05
−1.15
0.30
69.683
Inoculum size
0.017
0.03
0.80
0.46
54.235
Inoculum age
0.340
0.68
15.83
0.00
99.998
Agitation speed
−0.029
−0.06
−1.37
0.23
76.990
Medium volume
0.116
0.23
5.39
0.00
99.703
Starch
−0.140
−0.28
−6.52
0.00
99.873
NaNO3
0.040
0.08
1.85
0.12
87.656
K2HPO4
−0.031
−0.06
−1.45
0.21
79.328
MgSO4·7H2O
−0.145
−0.29
−6.76
0.00
99.892
Yeast extract
0.024
0.05
1.13
0.31
69.046
Malt extract
−0.109
−0.22
−5.07
0.00
99.615
Peptone
−0.186
−0.95
−8.65
0.00
99.966
Analysis of variance (ANOVA)
df
SS
MS
-test
Significance ( value)
Regression
14
5.485
0.3918
42.508
0.000305
Residual
5
0.0460
0.0092
Total
19
5.5312
: Student’s test; : corresponding level of significance; df: degree of freedom; SS: sum of squares; MS: mean sum of squares; : Fisher’s function; and significance : corresponding level of significance. Multiple 0.9958. square 0.9916. Adjusted square 0.9683.