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Journal of Nanomaterials
Volume 2016, Article ID 3439419, 7 pages
http://dx.doi.org/10.1155/2016/3439419
Research Article

Preparation, Characterization, and In Vitro and Vivo Antitumor Activity of Oridonin-Conjugated Multiwalled Carbon Nanotubes Functionalized with Carboxylic Group

Department of Pharmaceutical Engineering, Institute of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China

Received 5 December 2015; Revised 18 April 2016; Accepted 26 July 2016

Academic Editor: Ester Vazquez

Copyright © 2016 Chuanjin Wang and Wei Li. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Carbon nanotubes have shown great potential in tumor therapy. Oridonin (ORI) is a poorly water-soluble diterpenoid compound (C20H28O6) used in the treatment of esophageal and hepatic carcinoma for decades. For the purpose of enhancing the antitumor potency and reducing cytotoxicity of ORI, multiwalled carbon nanotubes functionalized with carboxylic group (MWCNTs-COOH) were used as ORI carrier. ORI was noncovalently encapsulated into (or onto) the functionalized carbon nanotubes (MWCNTs-ORI). The obtained MWCNTs-ORI has been characterized. The ORI loading efficiency in MWCNTs-COOH carrier was studied to be about 82.6% (w/w). In vitro cytotoxicity assay on MWCNTs-ORI gave IC50 of μg/mL and ORI-F gave IC50 of μg/mL. The antitumor effect studies in vivo showed that MWCNTs-ORI improved antitumor activity of ORI in comparison with ORI-F. The tumor inhibition ratio for MWCNTs-ORI ( g·Kg−1·d−1) was 86.4%, higher than that of ORI-F ( g·Kg−1·d−1) which was 39.2%. This can greatly improve the pharmaceutical efficiency and reduce potential side effects.