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Journal of Nanomaterials
Volume 2016, Article ID 3941501, 10 pages
Research Article

Carbon Nanotubes-Hydroxyapatite Nanocomposites for an Improved Osteoblast Cell Response

1Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania
2National Institute of Materials Physics, 105 Bis Atomistilor, 077125 Magurele, Romania
3Institut des Sciences de la Terre d’Orléans (ISTO), UMR 7327 CNRS, Université d’Orléans, 1A rue de la Férollerie, 45071 Orléans Cedex 2, France
4Chemical and Materials Engineering, University of Dayton Research Institute, 300 College Park, Dayton, OH 45469, USA

Received 29 December 2015; Accepted 7 March 2016

Academic Editor: Victor M. Castaño

Copyright © 2016 Sabrina Constanda et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


An alternative and simple coprecipitation method was developed to obtain carbon nanotube-hydroxyapatite (CNTs:HAp) based nanocomposites. The incorporation of CNTs (in a concentration of 5% and 10% of total weight of the nanocomposite) and their impact on both structural and biological properties were studied by using a set of standard complementary biological, microscopic, and spectroscopic techniques. The characteristic peaks of carbon structure in CNTs were not observed in the CNTs-HAp composites by X-ray diffraction analysis. Moreover, FTIR and Raman spectroscopies confirmed the presence of HAp as the main phase of the synthesized CNTs:HAp nanocomposites. The addition of CNTs considerably affected the nanocomposite morphology by increasing the average crystallite size from 18.7 nm (for raw HAp) to 28.6 nm (for CNTs:HAp-10), confirming their proper incorporation. The biocompatibility evaluation of CNTs:HAp-5 and CNTs:HAp-10 nanocomposites included the assessment of several parameters, such as cell viability, antioxidant response, and lipid peroxidation, on human G-292 osteoblast cell line. Our findings revealed good biocompatibility properties for CNTs:HAp nanocomposites prepared by the coprecipitation method supporting their potential uses in orthopedics and prosthetics.