Research Article
In Vitro Evaluation of Colloidal Silver on Immune Function: Antilymphoproliferative Activity
Figure 7
Cell viability of human macrophages, phagocytosis, and peroxynitrites production. Human macrophages (1 × 106 cells) were treated with AgC and incubated for 5 h at 37°C and 5% CO2 atmosphere. (a) Cell viability was analyzed by trypan blue assay. (b) Macrophages phagocytosis of FITC-Dextran evaluated by flow cytometry. (c) Nitrate/nitrite determined by Griess reaction (nitrate/nitrite colorimetric assay kit); the LPS was used as positive control. The data represent the mean ± standard deviation of three independent experiments. . AgC, colloidal silver; FITC-Dextran, fluorescein isothiocyanate-Dextran; and LPS, lipopolysaccharide.
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