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Journal of Nanomaterials
Volume 2016, Article ID 4360659, 12 pages
Research Article

Bioactive Glass Nanoparticles-Loaded Poly(ɛ-caprolactone) Nanofiber as Substrate for ARPE-19 Cells

1Department of Metallurgical and Materials Engineering, Federal University of Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil
2Faculty of Pharmacy, Federal University of Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil
3School of Pharmacy, Federal University of São João del-Rei, 35501-296 Divinópolis, MG, Brazil
4INSERM, U872, Team 17, Centre de Recherche des Cordeliers, 75006 Paris, France
5Université René Descartes Sorbonne Paris Cité, 75006 Paris, France
6Assistance Publique Hôpitaux de Paris, Hôtel-Dieu de Paris, 75004 Paris, France

Received 19 April 2016; Revised 10 June 2016; Accepted 22 June 2016

Academic Editor: Andrea Falqui

Copyright © 2016 Tadeu Henrique Lima et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Bioactive glass nanoparticles-loaded poly(ɛ-caprolactone) nanofibers (BIOG PCL nanofibers) were synthesized and evaluated as substrates for ocular cells (ARPE-19). BIOG PCL nanofibers were characterized using SEM, FTIR, and DSC, and the in vitro degradation profile was also investigated. The in vitro ocular biocompatibility of nanofibers was exploited in Müller glial cells (MIO-M1 cells) and in chorioallantoic membrane (CAM); and the proliferative capacity, cytotoxicity, and functionality were evaluated. Finally, ARPE-19 cells were seeded onto BIOG PCL nanofibers and they were investigated as supports for in vitro cell adhesion and proliferation. SEM images revealed the incorporation of BIOG nanoparticles into PCL nanofibers. Nanoparticles did not induce modifications in the chemical structure and semicrystalline nature of PCL in the nanofiber, as shown by FTIR and DSC. MIO-M1 cells exposed to BIOG PCL nanofibers showed viability, and they were able to proliferate and to express GFAP, indicating cellular functionality. Moreover, nanofibers were well tolerated by CAM. These findings suggested the in vitro ocular biocompatibility and absence of toxicity of these nanofibers. Finally, the BIOG nanoparticles modulated the protein adsorption, and, subsequently, ARPE-19 cells adhered and proliferated onto the nanostructured supports, establishing cell-substrate interactions. In conclusion, the biodegradable and biocompatible BIOG PCL nanofibers supported the ARPE-19 cells.