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Journal of Nanomaterials
Volume 2016, Article ID 5178029, 8 pages
Research Article

DNA Microspheres Coated with Bioavailable Polymer as an Efficient Gene Expression Agent in Yeasts

1Bar-Ilan Institute for Nanotechnology and Advanced Materials, Department of Chemistry, Bar-Ilan University, 52900 Ramat Gan, Israel
2Department of Materials Science & Engineering, National Cheng Kung University, Tainan 70101, Taiwan

Received 21 September 2015; Revised 18 November 2015; Accepted 26 November 2015

Academic Editor: Abdelwahab Omri

Copyright © 2016 Irena Reytblat et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Gene delivery is one of the steps necessary for gene therapy and for genetic modification. However, delivering DNA into cells is challenging due to its negative charge that leads to repulsion by the negative cell membrane. In the current research, DNA spheres with a DNA encoding to a certain gene were coated with bioavailable polymers, polyethylene imine (PEI) and polycaprolactone (PCL), in a short, one-step sonochemical reaction. The polymers were used in order to neutralize the negative charge of the DNA. Our study shows that the DNA nanospheres not only managed to penetrate the cell without causing it any damage, but also expressed the desired gene inside it.