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Journal of Nanomaterials
Volume 2016 (2016), Article ID 5217023, 11 pages
http://dx.doi.org/10.1155/2016/5217023
Research Article

Monitoring of Glucose in Beer Brewing by a Carbon Nanotubes Based Nylon Nanofibrous Biosensor

Faculty of Science and Technology, Free University of Bozen-Bolzano, Piazza Università 5, 39100 Bolzano, Italy

Received 25 March 2016; Revised 24 May 2016; Accepted 25 May 2016

Academic Editor: William Yu

Copyright © 2016 Marco Mason et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This work presents the design, preparation, and characterization of a novel glucose electrochemical biosensor based on the immobilization of glucose oxidase (GOX) into a nylon nanofibrous membrane (NFM) prepared by electrospinning and functionalized with multiwalled carbon nanotubes (CNT). A disc of such GOX/CNT/NFM membrane (40 μm in thickness) was used for coating the surface of a glassy carbon electrode. The resulting biosensor was characterized by cyclic voltammetry and chronoamperometry, with ferrocene methanol as mediator. The binding of GOX around the CNT/NFM greatly enhances the electron transfer, which results in a biosensor with a current five times higher than without CNT. The potential usefulness of the proposed biosensor was demonstrated with the analysis of glucose in commercial beverages and along the monitoring of the brewing process for making beer, from the mashing to the fermentation steps.