SPR Biosensing MUA/Poly-L-lysine Platform for the Detection of 2,4-Dinitrophenol as Small Molecule Model System

Figure 4

Sensorgrams showing the SPR signal resulting from the injection of anti-DNP solutions over BSA and BSA-DNP coated single and mixed SAMs. In (a) the SPR response of single MUA self-assembled monolayers to the passage of 750 nM anti-DNP is presented. In (b) the SPR change from MUA/DTT SAMs to the flow of 190 nM anti-DNP is displayed. The antibody solutions were injected during ten minutes followed by the injection of running buffer. Black and red curves give account of nonspecific antibody adsorption and total antibody binding, respectively. In the selected sensorgrams, nonspecific antibody binding gives account of 26% of the total antibody binding in single SAM and 48% of the total antibody binding in mixed SAMs.

(a)

(b)