Enhanced Stem Cell Osteogenic Differentiation by Bioactive Glass Functionalized Graphene Oxide Substrates

Figure 7

Semiquantitative analysis of GO/BG stimuli induced osteogenic differentiation of rBMSCs. (a) Immunostaining of osteogenic proteins BMP2, OPN, and RUNX2 on different samples at 7 days: (A) DGO, (B) 1 wt.% DGO/BG, (C) 5 wt.% DGO/BG, and (D) BG. Scale bars, 20 μm. (b) Quantification of mean immunofluorescence intensities of BMP2, OPN, and RUNX2 in groups as defined in (a). All measurements were performed in triplicate and analyzed by one-way ANOVA with Tukey’s posttest to account for multiple comparisons. Data were plotted as mean; error bars are SEM. BMP2, red; OPN, red; RUNX2, green (, ).

(a)

(b)