Research Article

Binding Affinity, Cellular Uptake, and Subsequent Intracellular Trafficking of the Nano-Gene Vector P123-PEI-R13

Figure 3

Effect of chlorpromazine hydrochloride (CPZ), glucose, and C9 on transfection efficiency of P123-PEI-R13/DNA complexes in Hela cells at w/w ratio of 30. CPZ and glucose were used as the inhibitors of clathrin-mediated endocytosis. C9 was the competitor of P123-PEI-R13/DNA complexes for clathrin-mediated endocytosis. The RLU/mg protein in control cells was set to 100%. Each data point represents the mean ± standard deviation (, ).