Research Article
Label-Free Detection of Chondroitin Sulphate Proteoglycan 4 by a Polyaniline/Graphene Nanocomposite Functionalized Impedimetric Immunosensor
Figure 2
Fabrication and characterization of polyaniline@graphene oxide (PANI@GO) immunosensor. (a) Electrochemical codeposition of PANI@GO on ITO electrode: cycle 1: current density of 0.6 mA⋅cm−2 for 3 min; cycle 2: a current density 0.3 mA⋅cm−2 for 15 min. The SEM morphology of the PANI@GO nanocomposite at each deposition circle. (b) The cyclic voltammograms of PANI@GO nanocomposite and bare ITO electrode (insert) scanning in 1 M NaCl with a scan rate of 50 mV/s. (c) Electrochemical impedance spectra of PANI@GO/ITO (A), glutaraldehyde activated PANI@GO/ITO (B), and CSPG4 specific mAbD2.8.5 immobilized PANI@GO/ITO.
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