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Journal of Nanomaterials
Volume 2016, Article ID 8932908, 13 pages
Research Article

One-Step Electrosynthesis of Graphene Oxide-Doped Polypyrrole Nanocomposite as a Nanointerface for Electrochemical Impedance Detection of Cell Adhesion and Proliferation Using Two Approaches

Yuan Li1,2 and Chao Yu1

1Institute of Life Science, Chongqing Medical University, Chongqing 40016, China
2Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China

Received 20 November 2015; Revised 8 January 2016; Accepted 1 June 2016

Academic Editor: Silvia Licoccia

Copyright © 2016 Yuan Li and Chao Yu. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A novel nanointerface of graphene oxide-doped polypyrrole (GO/PPy) is prepared on the surface of an indium tin oxide (ITO) electrode for electrochemical impedance detection of cell adhesion and proliferation through a facile one-step electropolymerization. The prepared GO/PPy nanocomposite had a robust surface and provided a biocompatible substrate for A549 cells adhesion and proliferation. The adhesion and proliferation of A549 cells on the surface of the GO/PPy modified ITO electrode directly increased the electron transfer resistance of [Fe(CN)6]3−/4− redox probe and influenced the impedance properties of the GO/PPy modified ITO electrode system. Based on these results, the adhesion and proliferation of A549 cells could be detected by electrochemical impedance technology using two approaches. Therefore, the present paper confirms that the GO/PPy nanocomposite film provides an excellent biological-electrical interface for cell immobilization and offers advantages of simple, low-cost fabrication and multiparameter detection and possesses potential application in cytological studies.