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Journal of Nanomaterials
Volume 2016, Article ID 8932908, 13 pages
http://dx.doi.org/10.1155/2016/8932908
Research Article

One-Step Electrosynthesis of Graphene Oxide-Doped Polypyrrole Nanocomposite as a Nanointerface for Electrochemical Impedance Detection of Cell Adhesion and Proliferation Using Two Approaches

Yuan Li1,2 and Chao Yu1

1Institute of Life Science, Chongqing Medical University, Chongqing 40016, China
2Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China

Received 20 November 2015; Revised 8 January 2016; Accepted 1 June 2016

Academic Editor: Silvia Licoccia

Copyright © 2016 Yuan Li and Chao Yu. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. H. N. Lim, N. M. Huang, S. S. Lim, I. Harrison, and C. H. Chia, “Fabrication and characterization of graphene hydrogel via hydrothermal approach as a scaffold for preliminary study of cell growth,” International Journal of Nanomedicine, vol. 6, pp. 1817–1823, 2011. View at Google Scholar · View at Scopus
  2. F. Song, X. Li, Q. Wang, L. Liao, and C. Zhang, “Nanocomposite hydrogels and their applications in drug delivery and tissue engineering,” Journal of Biomedical Nanotechnology, vol. 11, no. 1, pp. 40–52, 2015. View at Publisher · View at Google Scholar · View at Scopus
  3. Y. S. Gao, J. K. Xu, L. M. Lu et al., “Overoxidized polypyrrole/graphene nanocomposite with good electrochemical performance as novel electrode material for the detection of adenine and guanine,” Biosensors and Bioelectronics, vol. 62, pp. 261–267, 2014. View at Publisher · View at Google Scholar · View at Scopus
  4. D. R. Dreyer, S. Park, C. W. Bielawski, and R. S. Ruoff, “The chemistry of graphene oxide,” Chemical Society Reviews, vol. 39, no. 1, pp. 228–240, 2010. View at Publisher · View at Google Scholar · View at Scopus
  5. H. H. Zhou, G. Y. Han, Y. M. Xiao, Y. Z. Chang, and H.-J. Zhai, “Facile preparation of polypyrrole/graphene oxide nanocomposites with large areal capacitance using electrochemical codeposition for supercapacitors,” Journal of Power Sources, vol. 263, no. 1, pp. 259–267, 2014. View at Publisher · View at Google Scholar · View at Scopus
  6. Y. Mao, Y. Chen, S. Li, S. Lin, and Y. Jiang, “A graphene-based biosensing platform based on regulated release of an aptameric DNA biosensor,” Sensors, vol. 15, no. 11, pp. 28244–28256, 2015. View at Publisher · View at Google Scholar · View at Scopus
  7. H. Chen, J. Zhang, Y. Gao et al., “Sensitive cell apoptosis assay based on caspase-3 activity detection with graphene oxide-assisted electrochemical signal amplification,” Biosensors and Bioelectronics, vol. 68, pp. 777–782, 2015. View at Publisher · View at Google Scholar · View at Scopus
  8. F. Lin, X. Tong, Y. Wang, J. Bao, and Z. M. Wang, “Graphene oxide liquid crystals: synthesis, phase transition, rheological property, and applications in optoelectronics and display,” Nanoscale Research Letters, vol. 10, article 435, 2015. View at Publisher · View at Google Scholar · View at Scopus
  9. Y. Yang, H. Shi, Y. Wang et al., “Graphene oxide/manganese ferrite nanohybrids for magnetic resonance imaging, photothermal therapy and drug delivery,” Journal of Biomaterials Applications, vol. 30, no. 6, pp. 810–822, 2016. View at Publisher · View at Google Scholar · View at Scopus
  10. Y. Chang, S.-T. Yang, J.-H. Liu et al., “In vitro toxicity evaluation of graphene oxide on A549 cells,” Toxicology Letters, vol. 200, no. 3, pp. 201–210, 2011. View at Publisher · View at Google Scholar · View at Scopus
  11. M. Park, H. Choi, Y. Park, W. Lee, J. Lee, and M. Jeon, “Fabrication and characterization of graphene-based electrochemical sensors for glucose measurement,” Journal of Nanoscience and Nanotechnology, vol. 15, no. 10, pp. 7891–7894, 2015. View at Publisher · View at Google Scholar · View at Scopus
  12. D. Zhang, Y. Zhang, L. Zheng, Y. Zhan, and L. He, “Graphene oxide/poly-l-lysine assembled layer for adhesion and electrochemical impedance detection of leukemia K562 cancer cells,” Biosensors and Bioelectronics, vol. 42, no. 1, pp. 112–118, 2013. View at Publisher · View at Google Scholar · View at Scopus
  13. C. X. Guo, S. R. Ng, S. Y. Khoo, X. Zheng, P. Chen, and C. M. Li, “RGD-peptide functionalized graphene biomimetic live-cell sensor for real-time detection of nitric oxide molecules,” ACS Nano, vol. 6, no. 8, pp. 6944–6951, 2012. View at Publisher · View at Google Scholar · View at Scopus
  14. M. A. Booth, S. Harbison, and J. Travas-Sejdic, “Development of an electrochemical polypyrrole-based DNA sensor and subsequent studies on the effects of probe and target length on performance,” Biosensors and Bioelectronics, vol. 28, no. 1, pp. 362–367, 2011. View at Publisher · View at Google Scholar · View at Scopus
  15. J. G. Ayenimo and S. B. Adeloju, “Inhibitive potentiometric detection of trace metals with ultrathin polypyrrole glucose oxidase biosensor,” Talanta, vol. 137, pp. 62–70, 2015. View at Publisher · View at Google Scholar · View at Scopus
  16. J.-M. Moon, Y. Hui Kim, and Y. Cho, “A nanowire-based label-free immunosensor: direct incorporation of a PSA antibody in electropolymerized polypyrrole,” Biosensors and Bioelectronics, vol. 57, pp. 157–161, 2014. View at Publisher · View at Google Scholar · View at Scopus
  17. A. Fahlgren, C. Bratengeier, A. Gelmi et al., “Biocompatibility of polypyrrole with human primary osteoblasts and the effect of dopants,” PLoS ONE, vol. 10, no. 7, article e0134023, 2015. View at Publisher · View at Google Scholar
  18. D. D. Ateh, A. Waterworth, D. Walker, B. H. Brown, H. Navsaria, and P. Vadgama, “Impedimetric sensing of cells on polypyrrole-based conducting polymers,” Journal of Biomedical Materials Research. Part A, vol. 83, no. 2, pp. 391–400, 2007. View at Publisher · View at Google Scholar · View at Scopus
  19. Y. Li, G. L. Yuan, C. Y. Xia, and C. Yu, “Construction of a cell impedance biosensor based on polypyrrole-indium tin oxide micro-electrode for detecting cell biology behavior,” Chinese Journal of Analytical Chemistry, vol. 43, no. 12, pp. 1844–1850, 2015. View at Google Scholar
  20. W. Chen, Z. Lu, and C. M. Li, “Sensitive human interleukin 5 impedimetric sensor based on polypyrrole-pyrrolepropylic acid-gold nanocomposite,” Analytical Chemistry, vol. 80, no. 22, pp. 8485–8492, 2008. View at Publisher · View at Google Scholar · View at Scopus
  21. S.-H. Roh, “Electricity generation from microbial fuel cell with polypyrrole-coated carbon nanofiber composite,” Journal of Nanoscience and Nanotechnology, vol. 15, no. 2, pp. 1700–1703, 2015. View at Publisher · View at Google Scholar · View at Scopus
  22. S. Li, K. Shu, C. Zhao et al., “One-step synthesis of graphene/polypyrrole nanofiber composites as cathode material for a biocompatible zinc/polymer battery,” ACS Applied Materials and Interfaces, vol. 6, no. 19, pp. 16679–16686, 2014. View at Publisher · View at Google Scholar · View at Scopus
  23. P. A. Mini, A. Balakrishnan, S. V. Nair, and K. R. V. Subramanian, “Highly super capacitive electrodes made of graphene/poly(pyrrole),” Chemical Communications, vol. 47, no. 20, pp. 5753–5755, 2011. View at Publisher · View at Google Scholar · View at Scopus
  24. S. Biswas and L. T. Drzal, “Multilayered nanoarchitecture of graphene nanosheets and polypyrrole nanowires for high performance supercapacitor electrodes,” Chemistry of Materials, vol. 22, no. 20, pp. 5667–5671, 2010. View at Publisher · View at Google Scholar · View at Scopus
  25. C. Zhu, J. Zhai, D. Wen, and S. Dong, “Graphene oxide/polypyrrole nanocomposites: one-step electrochemical doping, coating and synergistic effect for energy storage,” Journal of Materials Chemistry, vol. 22, no. 13, pp. 6300–6306, 2012. View at Publisher · View at Google Scholar · View at Scopus
  26. Z. Lv, Y. Chen, H. Wei et al., “One-step electrosynthesis of polypyrrole/graphene oxide composites for microbial fuel cell application,” Electrochimica Acta, vol. 111, pp. 366–373, 2013. View at Publisher · View at Google Scholar · View at Scopus
  27. M. Deng, X. Yang, M. Silke et al., “Electrochemical deposition of polypyrrole/graphene oxide composite on microelectrodes towards tuning the electrochemical properties of neural probes,” Sensors and Actuators, B: Chemical, vol. 158, no. 1, pp. 176–184, 2011. View at Publisher · View at Google Scholar · View at Scopus
  28. Y. Hu, P. Zuo, and B.-C. Ye, “Label-free electrochemical impedance spectroscopy biosensor for direct detection of cancer cells based on the interaction between carbohydrate and lectin,” Biosensors and Bioelectronics, vol. 43, no. 1, pp. 79–83, 2013. View at Publisher · View at Google Scholar · View at Scopus
  29. I. Giaever and C. R. Keese, “Micromotion of mammalian cells measured electrically,” Proceedings of the National Academy of Sciences of the United States of America, vol. 88, no. 17, pp. 7896–7900, 1991. View at Publisher · View at Google Scholar · View at Scopus
  30. D. Schneider, M. Tarantola, and A. Janshoff, “Dynamics of TGF-β induced epithelial-to-mesenchymal transition monitored by Electric Cell-Substrate Impedance Sensing,” Biochimica et Biophysica Acta - Molecular Cell Research, vol. 1813, no. 12, pp. 2099–2107, 2011. View at Publisher · View at Google Scholar · View at Scopus
  31. J. Hong, K. Kandasamy, M. Marimuthu, C. S. Choi, and S. Kim, “Electrical cell-substrate impedance sensing as a non-invasive tool for cancer cell study,” Analyst, vol. 136, no. 2, pp. 237–245, 2011. View at Publisher · View at Google Scholar · View at Scopus
  32. X. Sun, J. Ji, D. Jiang et al., “Development of a novel electrochemical sensor using pheochromocytoma cells and its assessment of acrylamide cytotoxicity,” Biosensors and Bioelectronics, vol. 44, no. 1, pp. 122–126, 2013. View at Publisher · View at Google Scholar · View at Scopus
  33. C. K. Choi, A. E. English, S.-I. Jun, K. D. Kihm, and P. D. Rack, “An endothelial cell compatible biosensor fabricated using optically thin indium tin oxide silicon nitride electrodes,” Biosensors and Bioelectronics, vol. 22, no. 11, pp. 2585–2590, 2007. View at Publisher · View at Google Scholar · View at Scopus
  34. T. Patois, B. Lakard, S. Monney, X. Roizard, and P. Fievet, “Characterization of the surface properties of polypyrrole films: influence of electrodeposition parameters,” Synthetic Metals, vol. 161, no. 21-22, pp. 2498–2505, 2011. View at Publisher · View at Google Scholar · View at Scopus
  35. A. S. Karimullah, D. R. S. Cumming, M. Riehle, and N. Gadegaard, “Development of a conducting polymer cell impedance sensor,” Sensors and Actuators B: Chemical, vol. 176, pp. 667–674, 2013. View at Publisher · View at Google Scholar · View at Scopus
  36. L. L. Zhang, S. Zhao, X. N. Tian, and X. S. Zhao, “Layered graphene oxide nanostructures with sandwiched conducting polymers as supercapacitor electrodes,” Langmuir, vol. 26, no. 22, pp. 17624–17628, 2010. View at Publisher · View at Google Scholar · View at Scopus
  37. R. Balint, N. J. Cassidy, and S. H. Cartmell, “Conductive polymers: towards a smart biomaterial for tissue engineering,” Acta Biomaterialia, vol. 10, no. 6, pp. 2341–2353, 2014. View at Publisher · View at Google Scholar · View at Scopus
  38. M. Vandrovcová and L. Bačáková, “Adhesion, growth and differentiation of osteoblasts on surface-modified materials developed for bone implants,” Physiological Research, vol. 60, no. 3, pp. 403–417, 2011. View at Google Scholar
  39. C. Tlili, K. Reybier, A. Géloën et al., “Fibroblast cells: a sensing bioelement for glucose detection by impedance spectroscopy,” Analytical Chemistry, vol. 75, no. 14, pp. 3340–3344, 2003. View at Publisher · View at Google Scholar · View at Scopus
  40. C. Zhu, S. Guo, P. Wang et al., “One-pot, water-phase approach to high-quality graphene/TiO2 composite nanosheets,” Chemical Communications, vol. 46, no. 38, pp. 7148–7150, 2010. View at Publisher · View at Google Scholar · View at Scopus
  41. S. Bose, T. Kuila, M. E. Uddin, N. H. Kim, A. K. T. Lau, and J. H. Lee, “In-situ synthesis and characterization of electrically conductive polypyrrole/graphene nanocomposites,” Polymer, vol. 51, no. 25, pp. 5921–5928, 2010. View at Publisher · View at Google Scholar · View at Scopus
  42. R. Bissessur, P. K. Y. Liu, and S. F. Scully, “Intercalation of polypyrrole into graphite oxide,” Synthetic Metals, vol. 156, no. 16-17, pp. 1023–1027, 2006. View at Publisher · View at Google Scholar · View at Scopus
  43. A. Gelmi, M. J. Higgins, and G. G. Wallace, “Physical surface and electromechanical properties of doped polypyrrole biomaterials,” Biomaterials, vol. 31, no. 8, pp. 1974–1983, 2010. View at Publisher · View at Google Scholar · View at Scopus
  44. A. Gelmi, M. K. Ljunggren, M. Rafat, and E. W. H. Jager, “Influence of conductive polymer doping on the viability of cardiac progenitor cells,” Journal of Materials Chemistry B, vol. 2, no. 24, pp. 3860–3867, 2014. View at Publisher · View at Google Scholar · View at Scopus
  45. A. Venkatanarayanan, T. E. Keyes, and R. J. Forster, “Label-free impedance detection of cancer cells,” Analytical Chemistry, vol. 85, no. 4, pp. 2216–2222, 2013. View at Publisher · View at Google Scholar · View at Scopus
  46. L. Ding, C. Hao, X. J. Zhang, and H. X. Ju, “Carbon nanofiber doped polypyrrole nanoscaffold for electrochemical monitoring of cell adhesion and proliferation,” Electrochemistry Communications, vol. 11, no. 4, pp. 760–763, 2009. View at Publisher · View at Google Scholar · View at Scopus
  47. T. B. Tran, P. D. Nguyen, S. H. Um, S. J. Son, and J. Min, “Real-time monitoring in vitro cellular cytotoxicity of silica nanotubes using electric cell-substrate impedance sensing (ECIS),” Journal of Biomedical Nanotechnology, vol. 9, no. 2, pp. 286–290, 2013. View at Publisher · View at Google Scholar · View at Scopus
  48. K. Benson, S. Cramer, and H.-J. Galla, “Impedance-based cell monitoring: barrier properties and beyond,” Fluids and Barriers of the CNS, vol. 10, no. 1, article 5, 2013. View at Publisher · View at Google Scholar · View at Scopus