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Journal of Nanomaterials
Volume 2016 (2016), Article ID 9204573, 10 pages
Research Article

Green Synthesis of Silver Nanoparticles and Their Bactericidal and Antimycotic Activities against Oral Microbes

1Facultad de Odontologia, Universidad Autonoma de Nuevo Leon (UANL), 64460 Monterrey, NL, Mexico
2Facultad de Estomatologia, UASLP, 78290 San Luis Potosí, SLP, Mexico
3Facultad de Ciencias, UASLP, 78290 San Luis Potosí, SLP, Mexico

Received 11 November 2015; Revised 27 March 2016; Accepted 29 March 2016

Academic Editor: Paulo Cesar Morais

Copyright © 2016 Osvelia E. Rodríguez-Luis et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Nanotechnology is a new discipline with huge applications including medicine and pharmacology industries. Although several methods and reducing agents have been employed to synthesize silver nanoparticles, reactive chemicals promote toxicity and nondesired effects on the human and biological systems. The objective of this work was to synthesize silver nanoparticles from Glycyrrhiza glabra and Amphipterygium adstringens extracts and determine their bactericidal and antimycotic activities against Enterococcus faecalis and Candida albicans growth, respectively. 1 and 10 mM silver nitrate were mixed with an extract of Glycyrrhiza glabra and Amphipterygium adstringens. Green silver nanoparticles (AgNPs) were characterized by TEM, Vis-NIR, FTIR, fluorescence, DLS, TGA, and X-ray diffraction (XRD) analysis. Bactericidal and antimycotic activities of AgNPs were determined by Kirby and Bauer method and cell viability MTT assays. AgNPs showed a spherical shape and average size of 9 nm if prepared with Glycyrrhiza glabra extract and 3 nm if prepared with Amphipterygium adstringens extract. AgNPs inhibited the bacterial and fungal growth as was expected, without a significant cytotoxic effect on human epithelial cells. Altogether, these results strongly suggest that AgNPs could be an interesting option to control oral biofilms.