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Journal of Nanomaterials
Volume 2016, Article ID 9701870, 7 pages
Research Article

Improved Delivery of Caffeic Acid through Liposomal Encapsulation

1Postgraduate Institute of Science, University of Peradeniya, 20400 Peradeniya, Sri Lanka
2Sri Lanka Institute of Nanotechnology, Mahenwatte, Pitipana, 10200 Homagama, Sri Lanka
3Department of Chemistry, Faculty of Science, University of Peradeniya, 20400 Peradeniya, Sri Lanka
4Electrical Engineering Division, Department of Engineering, University of Cambridge, 9 JJ Thomson Avenue, Cambridge CB3 0FA, UK

Received 10 November 2015; Revised 3 February 2016; Accepted 16 February 2016

Academic Editor: Yasuhiko Hayashi

Copyright © 2016 Nuwanthi P. Katuwavila et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Photoageing resulting from long term exposure of the skin to UV light can be minimized by scavenging the reactive photochemical intermediates with antioxidants. For effective photoprotection, the antioxidant must overcome the barrier properties of the skin and reach the target site in significant amounts. The present study aims to improve the skin penetration of caffeic acid, a very effective free radical scavenger, by encapsulating in liposomes. Caffeic acid loaded liposomes prepared using the reverse phase evaporation technique showed 70% encapsulation efficiency and size around 100 nm with zeta potential of −55 mV. In vitro diffusion through a dialysis membrane enabled 70% release of encapsulated caffeic acid within 7 h, whereas 95% of free caffeic acid diffused within 4 h in PBS solution (pH 7.4). Liposomal caffeic acid permeation through pig skin epidermis in a Franz cell apparatus was 45 % during 7 h. In contrast, free caffeic acid was almost nonpermeable (<5%) to pig skin during this time. The DPPH assay indicated that skin penetration did not destroy the antioxidant activity of liposomal caffeic acid or free caffeic acid. In conclusion, we confirm that liposomal caffeic acid may be successfully employed as an effective photoprotective agent against UV mediated skin damage.