An Efficient Method for Cellulose Nanofibrils Length Shearing via Environmentally Friendly Mixed Cellulase Pretreatment

Figure 3

The XRD patterns of O-Pulp and enzymatic hydrolysis samples with different enzymatic charge and hydrolysis time. (a) The XRD patterns of the typical diffraction of crystal lattice type I with different conditions. The diffraction peaks were around 2θ = 16° and 22.5°. (b) The crystallinity of cellulose varied under different enzymatic hydrolysis pretreatments. With the cellulase charge increasing, the crystallinity exhibited a ladder increase due to the layer-by-layer hydrolysis. However, the crystallinity trended to evidently decrease with hydrolysis time, which suggested that cellulase hydrolysis rate of amorphous domains reduced gradually and was less than that of crystalline domains.

(a)

(b)