Design and Characterization of Endostatin-Loaded Nanoparticles for In Vitro Antiangiogenesis in Squamous Cell Carcinoma

Table 1

Box-Behnken experimental design for formulation variables and responses at pH 7.4 and 6.8, respectively.

Formulation	Variables			Responses
	Surfactant conc. (mg/mL)	TPP conc. (mg/mL)	Polymer conjugate conc. (mg/mL)	Size (nm)	Surface charge (mV)	Conductivity (mS/cm)	Polydispersity index
F1	1.00	0.1	1.5	65.07	+16.10	0.054	0.259
F2	0.10	0.1	1.5	84.22	+19.80	0.079	0.367
F3	0.10	0.2	2.5	173.9	+10.40	0.056	0.689
F4	1.00	0.3	1.5	97.38	−1.87	0.048	0.302
F5	0.55	0.2	1.5	300.10	+11.92	0.053	0.529
F6	0.55	0.2	1.5	295.10	+9.05	0.053	0.715
F7	0.55	0.1	2.5	163.60	+11.00	0.038	0.268
F8	0.55	0.3	0.5	169.10	+8.95	0.062	0.731
F9	0.55	0.1	0.5	125.20	−6.11	0.067	0.403
F10	1.00	0.2	2.5	125.50	+0.76	0.049	1.000
F11	0.55	0.3	2.5	56.28	+9.08	0.055	1.000
F12	1.00	0.2	0.5	329.10	−12.30	0.052	0.788
F13	0.10	0.2	0.5	127.21	−8.94	0.057	1.000
F14	0.10	0.3	1.5	363.10	−7.63	0.062	0.408
F15	0.55	0.2	1.5	310.20	+13.10	0.048	0.550