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Journal of Nanomaterials
Volume 2017 (2017), Article ID 3528295, 15 pages
Research Article

Effect of Surface Charge and Hydrophobicity Modulation on the Antibacterial and Antibiofilm Potential of Magnetic Iron Nanoparticles

1Microbiology and Immunology Department and Pharmaceutical Chemistry Department, Faculty of Pharmacy, Ahram Canadian University, Cairo, Egypt
2Department of Chemistry, School of Sciences & Engineering, American University in Cairo, New Cairo, Egypt

Correspondence should be addressed to Hassan Mohamed El-Said Azzazy

Received 18 January 2017; Accepted 24 May 2017; Published 12 September 2017

Academic Editor: Jean M. Greneche

Copyright © 2017 Rania Ibrahim Shebl et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary material 1: Change in color of MNPs as induced by changing their status from magnetite (a) to meghemite (b).

Supplementary material 2: XRD data of the prepared MNPs.

Supplementary material 3: (a) OA-MNPs (after washing with water and before wash with ethanol) showing OA-NPs not dispersed in water instead floating on water surface. (b) After successive washing with ethanol to remove excess OA from surface of MNPs; OA-MNPs are still floating and unable to disperse.

Supplementary information 4: Evaluation of the APTMS-MNPs method was performed by comparing the Zeta potential shift imparted on the MNPs. (a) Charge on surface of MNPs by the cold synthesis method (+25 mV). (b) Surface charge of particles prepared by the hot synthesis method (-11 mV). The cold synthesis method was more efficient to coat the MNPs with a surface positive charge.

Supplementary material 5: Growth inhibitory potential of MNPs on test bacteria 24 h post incubation with variable concentrations of MNPs. Subcultured S. aureus (ATCC-6538) and E. coli (ATCC-8739) were incubated with varying concentrations of APTMS-MNPs and OA-MNPs for 24 h at 37°C on a shaker (200 rpm) for 24 h. At the end of the incubation period, samples were obtained from each flask and 10 fold serially diluted in sterile saline. One hundred µL of each dilution as well as control were spread on the surface of 3 nutrient agar plates, incubated at 37°C for 24 h and the average numbers of colony forming units (CFU/mL) were counted. (a) and (c) showed the effect of APTMS-MNPs on S. aureus and E. coli. (b) and (d) showed the effect of OA-MNPs on S. aureus and E. coli.

Supplementary information 6: FTIR spectrum of control OA-MNPs before and after contact with S. aureus and E. coli. For E. coli (a) indicates the polysaccharide (900 -1200 cm-1) and (b) indicates the band attributed to primary amine (1640 – 1560 cm-1). For S. aureus, the peaks denoted by (c) and (d) represent the C=N and the C-H, respectively.

Supplementary material 7: FTIR spectra of MNPs, Arg-MNPs, CA-MNPs and A-MNPs. (*) represent characterizing bands.

  1. Supplementary Material