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Journal of Nanomaterials
Volume 2017, Article ID 3615707, 7 pages
Research Article

Carbon Nanoparticles Based Electrochemical Biosensor Strip for Detection of Japanese Encephalitis Virus

1Department of Chemistry, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia
2Institute of Health and Community Medicine, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia

Correspondence should be addressed to Suk Fun Chin; moc.liamg@nihcnufkus

Received 28 July 2017; Revised 27 October 2017; Accepted 13 November 2017; Published 18 December 2017

Academic Editor: Rajesh R. Naik

Copyright © 2017 Huat Choi Lai et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We reported a disposable and sensitive electrochemical biosensor strip based on carbon nanoparticles modified screen-printed carbon electrode (SPCE) for rapid and sensitive detection of Japanese Encephalitis Virus (JEV). Amino group functionalized carbon nanoparticles were prepared from preformed chitosan nanoparticles. Japanese Encephalitis Virus (JEV) antibody was immobilized onto the surfaces of carbon nanoparticles through amide bonds formation between amino groups of carbon nanoparticles and carboxylic groups of JEV antibody. The analytical performance of SPCE electrochemical biosensor strip was characterized using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). SPCE electrochemical biosensor strip exhibited a linear detection range of 1–20 ngmL−1 with a low detection limit of 0.36 ngmL−1 (at S/N = 3) for JEV, detection sensitivity was 0.024 ngmL−1 for JEV, and analysis results were obtainable within 10 minutes. The potential clinical application of this SPCE electrochemical biosensor strip was demonstrated by the detection of JEV in human serum.