Plant Explants Grown on Medium Supplemented with Fe₃O₄ Nanoparticles Have a Significant Increase in Embryogenesis

Figure 2

Scheme of plant calluses obtaining process. Flax seedlings were obtained from sterilized flax seeds and in case of experiment A were cut on explants and divided into control (without NPs) and experimental (with 0.5 mg/l, 1 mg/l, and 1.5 mg/l) groups to obtain calluses; in case of experiment B explants were grown on basal MS medium and after 5 weeks obtained calluses were transferred onto control (without NPs) and experimental (with 0.5 mg/l, 1 mg/l, and 1.5 mg/l) media for 4 weeks. Finally, DNA was extracted from both experiment calluses and RAPD analysis was carried out.