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Journal of Nanomaterials
Volume 2017, Article ID 4807064, 8 pages
Research Article

Evaluation and Optimization of Paper-Based SERS Substrate for Potential Label-Free Raman Analysis of Seminal Plasma

1Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou 350007, China
2Fujian Maternal and Child Health Hospital, Fuzhou 350001, China

Correspondence should be addressed to Zufang Huang; ude.uhj@81gnauhz

Received 16 April 2017; Revised 8 July 2017; Accepted 18 July 2017; Published 28 August 2017

Academic Editor: Rajesh R. Naik

Copyright © 2017 Zufang Huang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Characterization and optimization of paper SERS substrate were performed in detail, in which morphologies and distribution of silver nanoparticles (AgNPs) on the paper substrate pretreated with different concentrations of NaCl and the subsequent soaking with colloidal AgNPs for different period of time were evaluated. Our results show that both NaCl concentration and soaking time with AgNPs have a significant influence on SERS enhancement, showing that an optimal EF of 2.27 × 107 was achieved when the paper substrate was treated with 20 mM NaCl and one-hour soak with AgNPs. Moreover, seminal plasma (SP) was specifically selected to evaluate the performance of paper-based SERS substrate for potential clinical detection and diagnosis. The optimization of the paper SERS substrate demonstrates potential applications in reliable on-site detection of SP and clinical diagnosis of fertility-related diseases as well.