Heterogeneous Responses of Ovarian Cancer Cells to Silver Nanoparticles as a Single Agent and in Combination with Cisplatin

Figure 1

Physicochemical characterization and cytotoxicity of AgNPs. PVP-coated silver nanoparticles were dispersed in water or PBS (40 μg/ml), briefly sonicated, and incubated at room temperature for 1 hour. (a) Hydrodynamic diameter for AgNPs in water or PBS is shown. (b) -potential for AgNPs dispersed in water is shown. (c) Electron micrographs of AgNPs (scale bar = 100 nm) are shown. A2780, SKOV3, and OVCAR3 cells were seeded and allowed to attach overnight and then treated with (d) AgNP (0–1000 μg/ml) or (e) CDDP (0–25 μM) for 72 hours. Cell viability was assessed by MTT assay. Data is shown as percent viability compared to vehicle treated cells. (f) IC₅₀ values were determined from three independent experiments. All data is shown ± standard deviation.

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