Heterogeneous Responses of Ovarian Cancer Cells to Silver Nanoparticles as a Single Agent and in Combination with Cisplatin

Figure 2

Cell line differences in cytotoxicity after AgNP treatment are specific to exposure to and uptake of intact nanoparticles. (a) A2780 cells were seeded and allowed to attach overnight and then treated for 72 h with AgNPs or an equivalent volume of filtrate separated from AgNPs. (b) A2780 or (c) OVCAR3 cells were seeded and allowed to attach overnight and then treated with AgNP or AgNO₃ (dosed by mass of Ag) for 72 h. Viability was assessed by MTT assay and all data is shown as percent viability relative to vehicle treated cells ± standard deviation. (d) A2780 and OVCAR3 cells were seeded and allowed to attach overnight and then treated with AgNP (0–2000 μg/ml) for 6 h. Treatment medium was replaced with normal growth medium and the cells were allowed to recover for 72 h. Calculated IC₅₀ values are shown in the inset. (e) A2780 were treated as above with AgNP (0, 15 μg/ml) and cytochalasin D (cyto D, 37.5 nM) included in the 6-hour treatment. Cell viability was assessed by MTT assay and data is shown as percent viability relative to vehicle treated cells ± standard deviation. Significant differences are indicated: ; Student’s -test.

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