Heterogeneous Responses of Ovarian Cancer Cells to Silver Nanoparticles as a Single Agent and in Combination with Cisplatin

Figure 4

Quantification of glutathione and DNA damage in ovarian cancer cells before and after AgNP exposure. A2780, SKOV3, and OVCAR3 cells were seeded and allowed to attach overnight and then treated with AgNP (10 or 100 μg/ml) or vehicle for 24 hours. (a) Cells were assessed for reduced GSH and oxidized GSSG. Data are shown as fold relative to vehicle treated control for each cell line with the GSH/GSSG ratio ± SD inlaid. (b) Phosphorylated (S139) and total H2AX were determined by ELISA. Data is shown as phospho/total -H2AX relative to vehicle treatment ± standard deviation for individual cell lines. Significant differences are indicated: ; ; Student’s -test.

(a)

(b)