Interaction of Solid Lipid Nanoparticles and Specific Proteins of the Corona Studied by Surface Plasmon Resonance

Figure 5

Sensorgrams showing the SPR signal resulting from the injection of the solid lipid nanoparticles (a) P188, (b) P188+, (c) PVA, (d) CL, and (e) CM over immobilized BSA (sensor surface: Au-MUA-BSA). Each cycle included a ten-minute injection of the SLN solution followed by a washing step with running buffer (PBS pH 7.4). In order to remove the bound SLN from the sensor surfaces, 1 min regeneration steps with Triton X-100 were performed. SPR signal corresponding to each SLN was measured in a time in between the end of the SLN injection and the start of the regeneration step (around ~ 1250 s of each cycle, except CL nanoparticles that the SPR signal was measured at around 750 s).

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