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Journal of Nanomaterials
Volume 2017, Article ID 6802397, 10 pages
https://doi.org/10.1155/2017/6802397
Research Article

Evaluation of the Xanthan-Based Film Incorporated with Silver Nanoparticles for Potential Application in the Nonhealing Infectious Wound

1Department of Surgery, Jinling Hospital, Nanjing, China
2Medical School of Southeast University, Nanjing, China
3Medical School of Nanjing University, Nanjing, China

Correspondence should be addressed to Jianan Ren; moc.liamg@rnanaij

Received 29 April 2017; Accepted 18 July 2017; Published 27 August 2017

Academic Editor: Piersandro Pallavicini

Copyright © 2017 Jinjian Huang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Xanthan gum is a high molecular weight polysaccharide biocompatible to biological systems, so its products promise high potential in medicine. In this study, we crosslinked xanthan gum with citric acid to develop a transparent film for protecting the wound. Silver nanoparticles (AgNPs) are incorporated into the film to enhance the antimicrobial property of our biomaterial. This paper discussed the characteristics and manufacturing of this nanocomposite dressing. The safety of the dressing was studied using fibroblasts (L929) by the method of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and staining of ethidium homodimer (PI) and calcein AM. The bacterial inhibition test and application of the dressing to nonhealing wounds infected with methicillin-resistant S. aureus (MRSA) were performed to evaluate the antibacterial effects in vitro and in vivo, respectively. The results indicated that the dressing could restrict the formation of biofilms, reduce inflammatory reactions, and promote the angiogenesis of granulation tissues in infectious wounds. Therefore, this dressing has a great advantage over traditional clinical products especially when administered under the condition of infections or for the purpose of infection prevention.