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Journal of Nanomaterials
Volume 2017, Article ID 7374510, 9 pages
Research Article

Optimal Zn-Modified Ca–Si-Based Ceramic Nanocoating with Zn Ion Release for Osteoblast Promotion and Osteoclast Inhibition in Bone Tissue Engineering

1Department of Orthopaedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
2National Engineering Research Center for Nanotechnology, Shanghai 200241, China
3Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200003, China
4Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China

Correspondence should be addressed to Xiaojian Ye; moc.361@enipsey and Daixu Wei; moc.361@iewxddivad

Received 21 December 2016; Revised 21 January 2017; Accepted 30 January 2017; Published 9 March 2017

Academic Editor: Chuanfei Guo

Copyright © 2017 Jiangming Yu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We investigated the slow release of Zn ion (Zn2+) from nanocoatings and compared the in vitro response of osteoblasts (MC3T3-E1) and proosteoclasts (RAW 264.7) cultured on Ca2ZnSi2O7 nanocoated with different Zn/Ca molar ratios on a Ti-6Al-4V (i.e., Ti) substrate to optimize cell behaviors and molecule levels. Significant morphology differences were observed among samples. By comparing with pure Ti and CaSiO3 nanocoating, the morphology of Ca2ZnSi2O7 ceramic nanocoatings was rough and contained small nanoparticles or aggregations. Slow Zn2+ release from nanocoatings was observed and Zn2+ concentration was regulated by varying the Zn/Ca ratios. The cell-response results showed Ca2ZnSi2O7 nanocoating at different Zn/Ca molar ratios for osteoblasts and osteoclasts. Compared to other nanocoatings and Ti, sample Zn/Ca (0.3) showed the highest cell viability and upregulated expression of the osteogenic differentiation genes ALP, COL-1, and OCN. Additionally, sample Zn/Ca (0.3) showed the greatest inhibition of RAW 264.7 cell growth and decreased the mRNA levels of osteoclast-related genes OAR, TRAP, and HYA1. Therefore, the optimal Zn-Ca ratio of 0.3 in Ca2ZnSi2O7 ceramic nanocoating on Ti had a dual osteoblast-promoting and osteoclast-inhibiting effect to dynamically balance osteoblasts/osteoclasts. These optimal Zn-Ca ratios are valuable for Ca2ZnSi2O7 ceramic nanocoating on Ti-coated implants for potential applications in bone tissue regeneration.