Optimal Zn-Modified Ca–Si-Based Ceramic Nanocoating with Zn Ion Release for Osteoblast Promotion and Osteoclast Inhibition in Bone Tissue Engineering

Figure 7

Real-time quantitative PCR analysis of gene expression for osteoclastic marker genes in RAW 264.7 cells and osteogenic marker genes in MC3T3-E1 cells grown on Ti, Zn/Ca (0), Zn/Ca (0.1), Zn/Ca (0.3), and Zn/Ca (0.5) at day 7, respectively. ; ; for each sample. The -axis represented the relative expression () normalized to expression level. Osteoclastic marker genes: (a) osteoclast-associated receptor (OAR), (b) tartrate-resistant acid phosphatase (TRAP), and (c) hyaluronoglucosaminidase 1 (HYA1). Osteogenic marker genes: (d) alkaline phosphatase (ALP), (e) collagen type-1 (COL1), and (f) osteocalcin (OCN).

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