Research Article
Natural Transformation of Zinc Oxide Nanoparticles and Their Cytotoxicity and Mutagenicity
Figure 5
The contribution of Zn2+ in the supernatant fraction to cell survival. (a) The mutation potential of pristine and alkalized ZnO nanoparticles (NPs) at a concentration approaching the lethal concentration (LC100, pristine NPs: 15 μg/mL; NPs alkalized for 20 days: 15 μg/mL; NPs alkalized for 60 days: 30 μg/mL; and NPs alkalized for 120 day: 60 μg/mL) for 72 h is shown. (b) After high-speed centrifugation and membrane filtration, cells were treated with the NP supernatants at concentrations approaching the LC100 for 72 h. The NP suspension without centrifugation was used as a control. Bars: ±SD. indicates .
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