Study of the Dynamic Uptake of Free Drug and Nanostructures for Drug Delivery Based on Bioluminescence Measurements

Figure 6

Kinetic curve of bioluminescence produced from the reaction of liposomes of EPC : chol = 70 : 30 and monolayer cells of H1299-pGL3 within 10,000 s. (Luciferin-loaded liposomes were separated from nonencapsulated luciferin by passing the extruded liposomal solution through a column of Sephadex G-75. To further prepare the liposomes, the released luciferin from the liposomes was separated using centrifugal ultrafiltration units with a molecular cutoff of 3 KD at 2000 ×g, 15°C for 20 min prior to detection. Interval of the measure time was set at 1000 ms.)