Morphology, Aggregation Properties, Cytocompatibility, and Anti-Inflammatory Potential of Citrate-Stabilized AuNPs Prepared by Modular Ultrasonic Spray Pyrolysis

Figure 5

Cytocompatibility of AuC18 and AuC20 in culture with L929 cells. (a) Phase contrast microscopy of L929 cells cultivated with AuNPs 100 μg/mL for 24 h is shown. (b) The viability of cells cultivated with AuNPs (12.5–200 μg/mL) is shown, as determined by the Trypan blue exclusion test. The results obtained in each experiment were normalized to control (100%), and these are shown as mean viability ± SD ( independent experiments). (c) A representative experiment is shown on the assessment of apoptosis of L929 cells cultivated with AuNPs by Annexin-V/PI staining out of three experiments with similar results. (d) Relative metabolic activity of L929 cells cultivated with AuNPs (12.5–200 μg/mL) is shown, as determined by MTT assay. The results are presented as mean % MTT ± SD ( independent experiments) relative to control (100%). (e) Proliferation of L929 cells in the presence of AuNPs (12.5–200 μg/mL) was determined after 3-day cultures, by 3H-thymidine incorporation assay. The results are shown as mean CPM (counts per minute) ± SD ( experiments). compared to control.

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