Morphology, Aggregation Properties, Cytocompatibility, and Anti-Inflammatory Potential of Citrate-Stabilized AuNPs Prepared by Modular Ultrasonic Spray Pyrolysis

Figure 6

Internalization of AuC18 and AuC20 NPs by monocytes and their viability. (a) Representative images of monocytes cultivated in the presence or absence of AuNPs (50 μg/mL) for 24 h are shown. The black arrows point to the cells with accumulated AuNPs. (b) A representative flow cytometry analysis of internalization of AuNPs by monocytes is shown, in which the side scatter parameter (SSlog) was used as an indicator of internal complexity of the cells. (c) Summarized results obtained from such analyses are shown as mean SSlog%  ± SD of 3 independent experiments. compared to nontreated monocytes (d) Representative analysis of apoptosis of LPS-treated and nontreated monocytes cultivated with AuNPs for 24 h and then stained with Annexin-V-FITC and PI. (e) Summarized data on total Annexin-V⁺ expression is presented as mean%  ± SD of 3 independent experiments. compared as indicated.

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