Identification on Mantle Cell Lymphoma Using CD20 and CD5 Coupled Upconversion Fluorescent Nanoprobes

Figure 5

Bright field image (a) of Jeko-1 cells (20×, 1 bar = 20 μm) and their overlays of blue/green (b–d) double-labeling upconversion fluorescence under a 980 nm laser excitation (40×, 1 bar = 10 μm). The punctate blue/green upconversion fluorescence comes from CD20 and CD5 nanoprobes, showing the existence of CD20 and CD5 antigens on the Jeko-1 cells and contributing to the identification of mantle cell lymphoma from B-cell lymphoma.