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Journal of Nanomaterials
Volume 2018, Article ID 4706057, 8 pages
Research Article

Evaluation of the Genotoxic and Antigenotoxic Effects of Andiroba (Carapa guianensis Aublet) Oil and Nanoemulsion on Swiss Mice

1Laboratório de Citogenética, Centro de Estudos Avançados da Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil
2Laboratório de Genética Toxicológica, Universidade de Brasília, Brasília, DF, Brazil
3Instituto Federal de Educação, Ciência e Tecnologia do Goiás, Goiânia, GO, Brazil
4Laboratório de Investigação Sistemática em Biotecnologia e Biodiversidade Molecular, Universidade Federal do Pará, Belém, PA, Brazil
5Laboratório de Nanobiotecnologia, Universidade de Brasília, Brasília, DF, Brazil

Correspondence should be addressed to Cleusa Yoshiko Nagamachi; moc.liamg@ihcamaganasuelc

Received 2 September 2017; Revised 2 January 2018; Accepted 16 January 2018; Published 28 February 2018

Academic Editor: Enrico Bergamaschi

Copyright © 2018 Karina Motta Melo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The Carapa guianensis (andiroba) oil is commonly used by the Amazon population for medicinal purposes. The objective of this study was to determine the genotoxic and antigenotoxic potential of the andiroba oil (AO) and nanoemulsion (AN) using Swiss mice. Therefore, we used the comet assay and micronucleus test. The AO predominant compounds were oleic (39.13%), palmitic (33.22%), and linoleic (16.86%) acids. AN composition obeyed the surfactant/oil ratio of 0.69, and the Tween 80/Span 80 ratio was held at 0.9. Our results showed no cytotoxicity or genotoxicity in the mice treated with AO and AN alone. However, there was a significant reduction in the polychromatic erythrocytes (PCEs) numbers in all groups treated with doxorubicin (DOX), including those pretreated with AO and AN. Thus, the samples tested did not protect against DOX. On the other hand, our results showed a large increase in micronucleus (MN) formation when the mice were treated with DOX alone; these numbers were reduced when the animals were pretreated with AO and AN. The results indicate a protective effect of andiroba on MN formation and show no evidence of genotoxicity in mice.