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Journal of Nanomaterials
Volume 2018, Article ID 4719790, 10 pages
https://doi.org/10.1155/2018/4719790
Research Article

Efficient Delivery of Therapeutic siRNA with Nanoparticles Induces Apoptosis in Prostate Cancer Cells

1Department of Central Laboratory, China-Japan Union Hospital, Jilin University, Changchun, Jilin 130021, China
2College of Pharmacy, Jilin University, Changchun, Jilin 130021, China
3Department of Urology, The First Hospital of Jilin University, Changchun, Jilin 130021, China

Correspondence should be addressed to Kebang Hu; moc.621@uh_gnabek

Received 18 September 2017; Revised 28 January 2018; Accepted 17 February 2018; Published 15 April 2018

Academic Editor: Jorge Pérez-Juste

Copyright © 2018 Xupeng Mu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Gene silencing using small interfering RNA (siRNA) has shown significant potential in the treatment of cancer. Herein, we developed the lipid-polymer hybrid nanoparticles (PEG-LP/siRNA NPs) for siRNA delivery. The cell viability assay indicated that PEG-LP/siRNA NPs had negligible cell cytotoxicity. The cellular uptake efficiency of PEG-LP/siRNA NPs measured by flow cytometry was up to 94.4%. Importantly, in vitro gene knockdown experiments demonstrated that PEG-LP/siJnk-1 NPs could significantly downregulate the expression of Jnk-1 at both the mRNA and protein levels in DU145 cells. Gene knockdown of Jnk-1 could activate apoptosis in part by the mitochondrial pathway in DU145 cells. Moreover, the PEG-LP/siJnk-1 NPs could inhibit tumor growth in a DU145 xenograft murine model, suggesting its therapeutic promise in cancer therapy.