Topographical Guidance of PSC-Derived Cortical Neurons

Figure 4

Immunofluorescent staining of human PSC-derived cortical neurons, cultured for 15 days on flat, pillar, and grooved topographies. Neurons were stained with Hoechst 33258, and antibodies against the neuronal marker, β3-tubulin (TUJ1; red color), and the progenitor marker, NESTIN (green color). Scalebar represents 100 μm. Picture insets represent a three times magnified detail of the original picture.